A Cell-based Lab-on-a-chip as an Alternative Method for Testing Skin Irritations
نویسندگان
چکیده
The Lab-on-a-Chip (LoC) described here is designed for the complex analysis of cytotoxic effects of chemical substances imposed on human keratinocytes. This LoC operating in real-time will have promising applications in cosmetic and pharmaceutical industries. All parameters and conditions on the LoC were optimized with respect to practical aspects under semi-automated conditions. The system includes monitoring of the cells via microscopic supervision as well as detection of stress-induced green fluorescence protein (GFP) expression combined with an impedimetric readout for quantification of cell morphology.
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